Photoswitchable Molecular Communication between Programmable DNA-Based Artificial Membraneless Organelles
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION(2022)
摘要
Spatiotemporal organization of distinct biological processes in cytomimetic compartments is a crucial step towards engineering functional artificial cells. Mimicking controlled bi-directional molecular communication inside artificial cells remains a considerable challenge. Here we present photoswitchable molecular transport between programmable membraneless organelle-like DNA coacervates in a synthetic microcompartment. We use droplet microfluidics to fabricate membraneless non-fusing DNA coacervates by liquid-liquid phase separation in a water-in-oil droplet, and employ the interior DNA coacervates as artificial organelles to imitate intracellular communication via photo-regulated uni- and bi-directional transfer of biomolecules. Our results highlight a promising new route to assembly of multicompartment artificial cells with functional networks.
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关键词
Artificial Cells, Artificial Organelles, DNA, Intracellular Communication, Phase Transitions
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