Mimic microgravity effect on muscle transcriptome under ionizing radiation

Spaceflight imposes the risk of skeletal muscle atrophy for astronauts. Two main factors of a spaceflight that results in deleterious effects are microgravity and cosmic rays in outer space. To study spaceflight-induced muscle atrophy with ground-based models, we performed two models of microgravity, tail suspension and denervation, in a low dose radiation environment and studied transcriptional changes in rat soleus muscle using microarrays. Soleus muscle from rats in the denervation group had greater expression changes compared to that found in rats from the tail suspension group. However, there was a very similar pattern of expression of differentially expressed genes (DEGs) in both models. In total, we identified 144 differentially expressed genes common in both models. Our study yielded two main findings. First, a large number of genes involved in energy metabolism were transcriptionally suppressed including those involved in fatty acid transport and beta-oxidation, and oxidative phosphorylation. Second, slow-twitch contractile protein encoding genes were down-regulated while there was an up-regulation in the fast-twitch type transcription. These results were consistent with other spaceflight studies on the effects on muscle cells, hence showed the potential of our ground-based models in studying spaceflight effects. The genes that might be involved in spaceflight effects will serve as candidate genes for future studies in understanding the mechanism of spaceflight-induced muscle atrophy and result in the development of effective countermeasures.