Development of a droplet digital polymerase chain reaction (ddPCR) assay for the detection of Tomato brown rugose fruit virus (ToBRFV) in tomato and pepper seeds

Brenda Yazmín Vargas-Hernández,José Abrahán Pool-Ramírez,Leandro Alberto Núñez-Muñoz,Berenice Calderón-Pérez,Rodolfo De La Torre-Almaráz,Jesús Hinojosa-Moya,Beatriz Xoconostle-Cázares,Roberto Ruiz-Medrano

Journal of Virological Methods（2022）

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摘要

•The detection of ToBRFV by ddPCR in tomato and pepper seeds is highly sensitive.•ddPCR can discriminate ToBRFV infection from other tobamoviruses.•ddPCR could be applicable for the detection of other plant viruses.

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Tomato brown rugose fruit virus,Tomato,Capsicum seeds,ddPCR

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