Cold Tolerance of Immature Stages of Ceratitis capitata and Bactrocera dorsalis (Diptera: Tephritidae)

Bactrocera dorsalis (Hendel) is a new fruit fly pest of some fruit types in the north and north eastern areas of South Africa. In order to determine whether existing cold disinfestation treatment schedules for an indigenous fruit fly pest: Ceratitis capitata (Wiedemann) would be effective for B. dorsalis, cold tolerances of four immature stages of the two species were compared. Studies were done in an artificial carrot-based larval diet. The developmental rates of the immature stages of the two species in the carrot-based larval diet were first determined at a constant temperature of 26 degrees C. The developmental times for eggs and three larval stages were found to be similar for the two species. Incubation times of both species after egg inoculation were determined to be 0, 3, 4, and 6 d for obtaining egg, first larval, second larval, and third larval stages respectively for the cold treatment. At a test temperature of -0.6 degrees C, mortality rates of C. capitata eggs, first instars, second instars, and third instars were lower than those of B. dorsalis. These results demonstrate that the current cold treatment schedules for disinfestation of C. capitata can be used as equally or more efficacious treatments for B. dorsalis.