Neobacillus paridis sp. nov., an endophyte of Paris polyphylla Smith var . yunnanensis

A novel endophytic strain, designated YIM B02564 T , was isolated from the root of Paris polyphylla Smith var . yunnanensis obtained from Yunnan Province, southwest China. By using a polyphasic approach, cells of the strain were characterized as facultative anaerobic, Gram-positive and rod-shaped. The growth conditions of the strain were found to occur at 20–55 °C (optimum, 30 °C), pH 6.0–9.0 (optimum, pH 7.0). Strain YIM B02564 T can tolerate 2% NaCl concentration. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM B02564 T belonged to the genus Neobacillus and the 16S rRNA gene sequence similarity values of strain YIM B02564 T to the type strains of members of this genus ranged from 95.6 to 97.8%. The DNA G+C content of strain YIM B02564 T calculated from the whole genome sequence was 41.6 mol%. Values of the ANI and the dDDH between strain YIM B02564 T and its closely related Neobacillus species were below 77.9% and 21.5%. Strain YIM B02564 T contained MK-7 as the major menaquinone, iso-C 15:0 and anteiso-C 15:0 as the major fatty acids. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid and four unidentified lipids. It contained meso -diaminopimelic acid in the cell-wall peptidoglycan. On the basis of polyphasic analysis, strain YIM B02564 T could be differentiated genotypically and phenotypically from recognized species of the genus Neobacillus . The isolate therefore represents a novel species, for which the name Neobacillus paridis is proposed. The type strain is YIM B02564 T (= JCM 34668 T = CGMCC 1.18655 T ).