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Assessment of the Murine Tumor Microenvironment by Multiplex Immunofluorescence.

Methods in molecular biology (Clifton, N.J.)(2022)

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Abstract
We describe the staining methods used for simultaneous detection of tumor microenvironment components as well as the automated quantification methodologies. This method uses mouse formalin-fixed paraffin-embedded tissues and multiplex immunofluorescence (Multiplex IF) followed by multispectral imaging. Currently, this methodology has shown to have a valuable role in murine immunoprofiling, and can be useful when evaluating the changes incurred on the tumor microenvironment upon various immunopreventive strategies.
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Key words
Immune markers,Multiplex IF,Opal™,Quantification algorithms,Vectra®
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