Reliable cell retention of mammalian suspension cells in microfluidic cultivation chambers
bioRxiv (Cold Spring Harbor Laboratory)(2022)
Abstract
We present a new microfluidic trapping concept to retain randomly moving suspension cells inside a cultivation chamber. In comparison to previously published complex multilayer structures, we achieve cell retention by a thin PDMS barrier, which can be easily integrated into various PDMS-based cultivation devices. Cell loss during cultivation is effectively prevented while diffusive media supply is still ensured.
### Competing Interest Statement
The authors have declared no competing interest.
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Key words
mammalian suspension cells,reliable cell retention,cultivation
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