Polysome profiling followed by quantitative PCR for identifying potential micropeptide encoding long non- coding RNAs in suspension cell lines

Micropeptides are emerging as important regulators of various cellular pro-cesses. Long non-coding RNAs (lncRNAs) serve as a source of micropeptide-en-coding small reading frames. The techniques to detect micropeptides or trans-lating lncRNAs, such as mass spectrometry and ribosome profiling, are sophisticated and expensive. Here, we present an easy and cost-effective proto-col to screen for potential micropeptide-encoding lncRNAs by polysome profiling in suspension cell lines. When combined with quantitative PCR, this pro-tocol facilitates the identification of a number of translating lncRNAs simulta-neously.For complete details on the use and execution of this protocol, please refer to Sun et al. (2021).