Analysis of sodium 2-mercaptoethane sulfonate in rat plasma using high performance liquid chromatography tandem-mass spectrometry

•MESNA is a drug used to combat the toxic effects of the anti-cancer agents: cyclophosphamide and ifosfamide.•A simple and effective method of analysis for MESNA in plasma via HPLC-MS/MS was developed and validated.•The method has a limit of detection of 20 nM and showcases wide linear range (0.05–200 μM).•The method was used to detect MESNA from the plasma of MESNA-treated rats.