Lactose-binding lectin from Vatairea macrocarpa seeds induces in vivo angiogenesis via VEGF and TNF-a expression and modulates in vitro doxorubicin-induced

Lactose-binding lectin from Vatairea macrocarpa seeds (VML) has attracted great attention due to its interesting biological activities, such as pro-inflammatory effects and macrophage activation. This study evaluated the cytotoxicity and genotoxicity/antigenotoxicity of VML in human lymphocytes using the CometChip assay, and angiogenic activity by the chick embryo chorioallantoic membrane (CAM) assay. In genotoxicity, lymphocytes were treated with different concentrations of VML (0.5, 2 and 8 mu M). In antigenotoxicity, lymphocytes were treated with the same concentrations of VML concomitant doxorubicin (90 mu M DXR). To evaluate angiogenesis, all CAM were treated with different concentrations of VML (0.5, 2 and 8 mu M) alone or co-treated with lactose (0.1 M). Furthermore, the levels of vascular endothelial growth factor (VEGF) and tumor necrosis factor-alpha (TNF-alpha) in CAM were assessed by immunohistochemistry. The results showed that VML was cytotoxic to lymphocytes, genotoxic at the highest concentration (8 mu M) and antigenotoxic at low concentrations (0.5, and 2 mu M). Regarding the CAM assay and immunohistochemistry, VML was angiogenic and significantly increased VEGF and TNF-alpha levels. In contrast, co-treatment with lactose significantly reduced the angiogenic effect and VEGF levels. We propose that protein-carbohydrate interactions between VML and glycans in the cell membrane are probably the major events involved in these activities. It seems likely that VML elicits a pro-inflammatory response through VEGF and TNF--alpha expression, resulting in increased vascularization at the site of inflammation. Therefore, our results show novel information on the effects of VML on DNA, as well as provide data regarded the neovascularization process involving this lectin. (C)& nbsp;2021 Elsevier B.V. and Societe & nbsp;Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.