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功劳叶提取物的体外抗菌活性研究

Chinese Journal of Veterinary Medicine(2016)

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Abstract
采用水提法和乙醇回流加热法分别提取功劳叶,应用牛津杯法和试管二倍稀释法分别检测功劳叶提取物对试验菌的抑菌圈直径、最低抑菌浓度(Minimum Inhibitory Consistency,MIC)和最低杀菌浓度(Minimum Bactericidal Consistency,MBC).结果显示,功劳叶醇提物金黄色葡萄球菌和大肠杆菌的抑菌圈直径分别为20.69 mm、15.40 mm;MIC值分别是31.3 mg/mL、125 mg/mL;MBC值分别是62.5 mg/mL、>500 mg/mL.功劳叶水提物对金黄色葡萄球菌的抑菌圈13.54mm,其MIC值、MBC值分别是62.5 mg/mL、500 mg/mL,对大肠杆菌抗菌活性较弱,其MIC值为500 mg/mL,MBC> 500 mg/mL.功劳叶不同提取物对细菌的抗菌活性有所差异,功劳叶醇提物对金黄色葡萄球菌和大肠杆菌均由不同程度的抑菌或杀菌能力.
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