Progress of Single-cell Metabolite Analysis Technology Based on Microfluidic System

Due to the heterogeneity between different cells , the analysis of metabolites at single cell level can provide abundant information for the study of complex biological systems. Microfluidic systems can manipulate fluids in microscale structures that are close to the size of cells, making microfluidic systems suitable for single-cell analysis. In this review, current single-cell metabolite analysis technology based on microfluidic systems was introduced. Various microfluidic single-cell metabolite analysis systems based on different detection techniques including fluorescence microscopic imaging, laser induced fluorescence, electrochemistry , mass spectrometry , chemiluminescence , sensor detection technology , as well as their applications were reviewed.