miR-424-5p通过靶向抑制HMGA1表达提高宫颈癌放射敏感性
Chinese Journal of Radiation Oncology(2021)
Abstract
目的:探讨miR-424-5p对宫颈癌放射敏感性的影响及作用机制。方法:RT-qPCR检测miR-424-5p在宫颈癌组织和Hela细胞中表达;流式细胞术检测Hela细胞凋亡率;CCK-8检测Hela细胞增殖活性;蛋白印迹法检测Hela细胞中蛋白表达水平。结果:相较于正常组织和细胞,宫颈癌组织和Hela细胞中miR-424-5p表达量降低(1.03∶0.88, P<0.01;1.00∶0.75, P<0.001)。过表达miR-424-5p会抑制经放射处理后Hela细胞增殖活性( P<0.01),同时增加放射处理后Hela细胞凋亡率(24.82%∶49.94%, P<0.001)。过表达miR-424-5p会抑制HMGA1表达(1.01∶0.63, P<0.01),miR-424-5p会直接作用HMGA1进而影响宫颈癌放射敏感性。 结论:miR-424-5p通过直接靶向HMGA1提高宫颈癌放射敏感性。
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Key words
microRNA,Wnt/β-catenin signaling pathway,Radiosensitivity,Cervical cancer
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