笔筒树孢子无菌培养体系的构建
Molecular Plant Breeding(2021)
Abstract
通过笔筒树孢子的无菌培养试验,以筛选出笔筒树孢子无菌培养各过程的最佳条件,建立一套系统、高效、完整的笔筒树的无菌培养技术体系,最终实现笔筒树种苗的工厂化生产.本研究用即采的成熟的离叶黑褐色孢子为材料,用75%酒精处理10s后再用0.1%升汞处理6 min结合进行笔筒树孢子的表面消毒,诱导孢子萌发的最适培养基为1/2 MS+6-BA 0.1 mg/L+IBA 0.2 mg/L,增殖培养基为MS+6-BA 0.05 mg/L+IBA 0.05 mg/L,分化培养基为MS+6-BA 0.2 mg/L+IBA 0.2 mg/L,生根培养基为MS+6-BA 0.05 mg/L+IBA0.05 mg/L,培养温度(25±2)℃,日光灯光源,光照时间12 h/d,光照强度2 000 lx.
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