油茶根际土壤解钾菌的发酵培养
Journal of Fujian Forestry Science and Technology(2021)
Abstract
采用双层平板法对已分离出的15株油茶根际解钾细菌进行筛选,获得3株解钾能力较强的菌株CoPDB 6、CoPDB 13和CoPDB 15,并对3个菌株的培养基、pH、装液量、接种量、温度和摇床转速等发酵培养条件进行优化.结果 表明,解钾细菌的最佳发酵培养条件为培养基A(蔗糖5.0g,磷酸氢二钠0.5g,云母粉1.0g,MgSO4·7H2O 0.5 g,氢化铁0.005 g,水1000 mL)、培养温度30℃、pH值7.2 ~7.4、装液量20% ~ 30% (50 ~ 75 mL/250 mL)、接种量9%、摇床转速200 r·min-1.
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