甘肃省5种昆虫病原线虫对草地贪夜蛾的致病力测定
Pratacultural Science(2021)
Abstract
探讨甘肃省昆虫病原线虫对草地贪夜蛾(Spodoptera frugiperda)的防治效果.通过室内生物测定研究了甘肃省5种10个昆虫病原线虫品系对草地贪夜蛾2龄幼虫的致病力,并用筛选出的高效品系对预蛹和蛹进行致病力测定.结果表明,夜蛾斯氏线虫(Steinernema feltiae)?0663PG和卡森斯氏线虫(Steinernema krussei)?0657L对草地贪夜蛾2龄幼虫有较高的致病力.在线虫和2龄幼虫数量比为160:1时,夜蛾斯氏线虫0663PG和卡森斯氏线虫0657L侵染72?h后,草地贪夜蛾死亡率分别达到100%和80%.草地贪夜蛾预蛹在夜蛾斯氏线虫0663PG的处理下2?d后均可成功化蛹,处理15?d后在数量比为3200:1时,蛹的羽化率为96.67%.数量比为12800:1时,夜蛾斯氏线虫0663PG侵染草地贪夜蛾蛹13?d后,蛹的羽化率为93.33%,夜蛾斯氏线虫0663PG对草地贪夜蛾预蛹和蛹的致死率均低于10%.综上可知,夜蛾斯氏线虫0663PG线虫品系对草地贪夜蛾幼虫具有较好的防治效果.草地贪夜蛾预蛹和蛹对昆虫病原线虫不敏感,线虫侵染后对蛹的羽化率和羽化时间没有显著影响(P?>?0.05).
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