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水稻稻瘟病病原菌分离纯化及分子鉴定

Hubei Agricultural Sciences(2021)

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Abstract
从牡丹江市不同水稻栽培地采集疑似感染稻瘟病的水稻叶片,用PDA培养基进行病原菌分离纯化培养,通过观察菌落黑色素及分生孢子的形态进行初筛鉴别;采用试剂盒提取菌丝DNA,利用稻瘟病菌特异引物进行PCR扩增,对扩增产物进行测序及Nucleotide Blast比对.结果表明,6个栽培区采集病样经初筛确定5株有黑色素分泌且观察到其分生孢子,测序证实其中2个样品PCR产物序列相同,且与NCBI网站公布的半知菌亚门的灰梨孢(Pyricularia grisea)高度同源,确定获得稻瘟病菌纯菌株.
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