A method for the quantitative determination of urinary estriol conjugates in human pregnancy

Journal of Steroid Biochemistry(1972)

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摘要
A new gas-liquid Chromatographic method for the quantitative determination of estriol-3-glucuronide, estriol-3-sulfate,16-glucuronide, estriol-16-glucuronide and estriol-3-sulfate in human pregnancy urine is described. The accuracy of the method was improved by the use of both labeled and nonlabeled internal standards in every determination. Data on the accuracy, specificity, precision and sensitivity of the new method indicate that it fulfills the reliability criteria and is suitable for quantitative analysis of urinary estriol conjugates in human pregnancy.
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关键词
Estriol-3-glucuronide (E3-3Gl) = 16α, 17β-dihydroxyestra-1,3,5(10)-trien-3-yl-β-D-glucopyranosiduronic acid,estriol-3-sulfate,16-glucuronide (E3-3S, 16Gl) = 17β-hydroxyestra-1,3,5(10)-trien-3-yl-sulfate-16α-yl-β-D-gluco-pyranosiduronic acid,estriol-16-glucuronide (E3-16Gl)= 3,17β-dihydroxyestra-1,3,5(10)-trien-16α-yl-β-D-glucopyranosiduronic acid,estriol-3-sulfate (E3-3S) = 16α,17β-dihydroxyestra-1,3,5(10)-trien-3-yl-sulfate,estriol-17-glucuronide (E3-17Gl) = 3,16α-dihydroxyestra-1,3,5(10)-trien-1 17β-yl-β-D-glucopyranosiduronic acid,estriol-3.17-disulfate (E3-3.17-diS) = 16α-hydroxyestra-1.3.5(10)-trien-3.17β-yl-disulfate.
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