一种快速检测Hg2+的比率荧光传感器构建
Food Science(2021)
Abstract
以汞离子(Hg2+)为检测对象,构建一种基于(silicon-doped carbon quantum dots,Si-CDs)-溶菌酶(lysozyme,Lys)功能化的金纳米簇的比率型荧光传感器.以发蓝色荧光的Si-CDs为参比荧光信号,发红色荧光的Lys功能化金纳米簇(Lys-AuNCs)为响应荧光信号构建比率荧光探针.基于Au+-Hg2+间的亲金效应,Hg2+可高效猝灭Lys-AuNCs在670?nm波长处的荧光发射,而Si-CDs在470?nm波长处的荧光强度保持不变,其荧光强度比值(I670/I470)与Hg2+(0~0.016?mg/L)呈良好的线性关系,线性方程为I670/I470=1.35-64.18C(Hg2+).对湖水、矿泉水、自来水3个水样进行添加回收实验,其回收率均在94.3%~115.0%之间,检出限为0.001?mg/L(3σ),变异系数不大于10.3%,且并无其他金属离子干扰.
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