Capture-SELEX技术筛选β-苯乙胺核酸适配体
Journal of Chinese Institute of Food Science and Technology（2021）
Abstract
利用指数富集配体的系统进化技术(SELEX)中基于固定ssDNA文库的捕获SELEX(Capture-SELEX)技术,筛选能特异性识别β-苯乙胺的核酸适配体.通过在Capture-SELEX技术中结合熔解曲线监测ssDNA文库的富集,并经过氧化石墨烯(GO)荧光法验证候选适配体的亲和力和特异性,证实获得一条特异性识别β-苯乙胺的适配体(PHE-2),解离常数(Kd)为(71.64±11.47)nmol/L.该适配体为β-苯乙胺的分析检测提供了新的分子识别元件.
MoreKey words
capture-selex
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