藁本黄酮的纯化及体外抗氧化活性研究
Applied Chemical Industry（2021）
Abstract
研究了藁本黄酮纯化的工艺条件及纯化前后藁本黄酮的体外抗氧化活性.结果表明,以D-101大孔树脂纯化藁本黄酮的最佳条件为:体积2.5 BV、浓度2.5 mg/mL、pH 4的上样液以3 mL/min流速上样后,用体积5 BV、浓度70％的乙醇溶液以2 mL/min流速洗脱,此条件下藁本黄酮回收率可达81.88％,样品纯度达到71.09％.纯化后藁本黄酮的抗氧化性明显高于纯化前,其清除DPPH自由基、超氧阴离子自由基和羟基自由基的IC50值分别为386.8,764.0,953.3μg/mL.
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