牦牛蹄筋胶原蛋白提取工艺优化

Food Research and Development(2021)

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Abstract
为高效制备牦牛蹄筋胶原蛋白,以牦牛蹄筋为原料,采用热水法和酶解法提取牦牛蹄筋胶原蛋白,选择合适的提取方法,通过单因素试验分析料液比、温度、时间、加酶量对牦牛蹄筋胶原蛋白得率、提取率及纯度的影响,以正交试验优化酶解条件.结果表明:酶解法提取牦牛蹄筋胶原蛋白效果较好,最佳提取条件为料液比1:12(g/mL)、加酶量2.5%、温度43℃、时间11 h,胶原蛋白的得率、提取率及纯度分别为14.91%,69.94%和89.44%.
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