紫外分光光度法快速测定废菌丝体中麦角甾醇的含量
Sichuan Food and Fermentation(2021)
Abstract
为了快速测定废菌丝体中麦角甾醇的含量,本文对紫外分光光度法进行了考察.以二氯甲烷为提取溶剂,麦角甾醇在0.005~0.025 mg/mL范围内线性关系良好,相关系数为0.999 79,平均回收率为98.87%~103.65%,RSD值为2.03%~3.07%,样品在10h内稳定.本方法具有设备操作简便、检测快速的特点,适用于即时检测,在安全性、精密度和稳定性方面良好,可用于废菌丝体中麦角甾醇的快速测定.
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