分子印迹固相萃取-超高效液相色谱-串联质谱法测定牛奶中11种氨基糖苷类抗生素
Journal of Food Safety & Quality(2021)
Abstract
目的 建立分子印迹固相萃取-超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)检测牛奶中11种氨基糖苷类抗生素(巴龙霉素、妥布霉素、庆大霉素、卡那霉素、阿米卡星、安普霉素、链霉素、双氢链霉素、新霉素、壮观霉素、潮霉素B)残留的测定方法.方法 样品经5%三氯乙酸-乙腈提取,氨基糖苷类分子印迹聚合物(molecular imprinted polymers,MIPs)固相萃取柱净化,SiELC Obelisc R色谱柱分离,采用超高效液相色谱-串联质谱仪进行测定.结果 方法检出限为1.0~5.0 μg/kg,定量限为2.0~10.0 μg/kg.11种氨基糖苷类药物在1.0~500.0 μg/kg范围内关系良好(r2≥0.9911),牛奶中加标回收率为70.7%~115.6%,相对标准偏差(relative standard deviations,RSDs)(n=6)为1.1%~9.8%.结论 本方法准确度、灵敏度高,可用于牛奶中11种氨基糖苷类抗生素的同时检测.
MoreAI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined