Step 2: RNA extraction and RT-qPCR v1

Peter H L Krijger,Tim A Hoek,Sanne Boersma,Lieke I P M Donders,Maaike M C Broeders,Mark Pieterse, Pim W Toonen,Ive Logister,Bram M P Verhagen,Marjon J A M Verstegen,Thomas W van Ravesteyn,Rene J T M Roymans,Francesca Mattiroli,Jo Vandesompele,Monique Nijhuis, Stefan Meijer,Anton van Weert, Edwin Dekker,Fred J Dom,Rob Ruijtenbeek, Lieven B J van der Velden,Jeroen H B van de Bovenkamp, Martijn Bosch,Wouter de Laat,Marvin E Tanenbaum

semanticscholar（2021）

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Abstract

STRIP is a start-to-end streamlined and automated procedure for COVID-19 testing, centering on a single Tecan Fluent liquid-handling robot that can process over 14,000 samples per day. Key features of the customized Tecan Fluent robot are (1) on-board 1D and 2D barcode scanners, (2) an automated tube decapper, (3) two robotic arms for simultaneous processing of different procedural steps, (4) a newly-designed spin vessel to keep magnetic beads in solution and immediately transferable to 384-well plates, (5) a built-in magnetic deck and a 384-channel pipetting head for rapid RNA isolation, (6) a heating device for fast drying of RNA prior to elution, (7) a built-in plate sealer and (8) a plate storage system to allow processing of multiple sample plates in a single run (See Materials). Here we describe the RNA extraction and RT-qPCR protocol as currently applied in STRIP.

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