Biosynthesis of D-Mannitol by Diplodia pinea Biossíntese de D-Manitol por Diplodia pinea Biosíntesis de D-Manitol por Diplodia pinea

Paula Francislaine Moura ORCID: https://orcid.org/0000-0003-1617-3144 Universidade Federal do Paraná, Brazil E-mail: paulafrancislaine19@gmail.com Caroline Gribner ORCID: https://orcid.org/0000-0002-0179-1284 Universidade Federal do Paraná, Brazil E-mail: carol_gribner@yahoo.com.br Katlin Suellen Rech ORCID: https://orcid.org/0000-0001-6699-2450 Universidade Federal do Paraná, Brazil E-mail: katlinrech@gmail.com Larissa Junqueira Gatto ORCID: https://orcid.org/0000-0001-6451-7445 Universidade Federal do Paraná, Brazil E-mail: lari.gatto@gmail.com Ana Angélica Ruscheweyh Rigoni ORCID: https://orcid.org/0000-0001-9986-4228 Universidade Positivo, Brazil E-mail: rigoni.aar@gmail.com Fernando Cesar Martins Betim ORCID: https://orcid.org/0000-0002-1668-8626 Universidade Federal do Paraná, Brazil E-mail: fernandobetim@hotmail.com Ricardo Wagner ORCID: https://orcid.org/0000-0002-9100-4757 Universidade Federal do Paraná, Brazil E-mail: ricardo.wagner@ufpr.br Josiane de Fátima Gaspari Dias ORCID: https://orcid.org/0000-0002-8548-8505 Universidade Federal do Paraná, Brazil E-mail: josianefgdias@gmail.com Obdulio Gomes Miguel ORCID: https://orcid.org/0000-0002-2231-9130 Universidade Federal do Paraná, Brazil E-mail: obdulio@ufpr.br Celso Garcia Auer ORCID: https://orcid.org/0000-0002-4916-2460 Empresa Brasileira de Pesquisa Agropecuária Florestas, Brazil E-mail: celso.auer@gmail.com Marilis Dallarmi Miguel ORCID: https://orcid.org/0000-0002-1126-9211 Universidade Federal do Paraná, Brazil E-mail: marilisdmiguel@gmail.com Abstract Research with fungi in search of substances with potential biological activity is gaining more prominence since they are sustainable sources of new molecules. Diplodia pinea is a phytopathogenic fungus found in forest species, which has shown few reports of molecules with biological activity and may present promising biotechnological potential. Thus, the objective of this study was to detect substances produced by this fungus in a liquid medium, under stressful conditions. A metabolite was detected in the alcoholic fraction characterized by X-ray diffraction as D-mannitol. This is the first description of this monosaccharide produced by D. pinea.