Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19

Gubio Campos,Silvia Sardi,Melissa Falcao,Emilia Belitardo,Danilo Rocha,Carolina Rolo,Aline Menezes,Carina Pinheiro,Rejane Carvalho,João Almeida,Eric Aguiar,Luis Pacheco

SciELO (SciELO Preprints)（2020）

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摘要

Herein, we describe the detection of a SARS-CoV-2 genome through metatranscriptome next-generation sequencing directly from the nasopharyngeal swab of a suspected case of local transmission of Covid-19, in Brazil. Depletion of human ribosomal RNA and use of an optimized in-house developed bioinformatics strategy contributed to successful detection of the virus.

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nasopharyngeal swab metatranscriptomics,torrent-based

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