Molecular spikes: a gold standard for single-cell RNA counting

bioRxiv (Cold Spring Harbor Laboratory)(2021)

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摘要
AbstractMolecule counting is central to single-cell sequencing, yet no experimental strategy to evaluate counting performance exists. Here, we introduce molecular spikes, novel RNA spike-ins containing inbuilt unique molecular identifiers that we use to identify critical experimental and computational conditions for accurate RNA counting across single-cell RNA-sequencing methods. The molecular spikes are a new gold standard that can be widely used to validate RNA counting in single cells.
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关键词
rna,molecular spikes,single-cell
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