Antibiogram studies and screening for ESβL (Extended Spectrum Beta Lactamase) producing E. coli strains isolated from clinical urine samples

Bacteria are capable of invading and infecting humans, leading to disease and sometimes death. Different body organs and tissues are vulnerable to different organisms. This study was aimed to isolate and identify the aerobic bacteria causing Urinary Tract Infection in different age groups. The mid-stream urine samples showing symptoms of urinary tract infections were collected from different hospitals and diagnostic centres of Kalaburagi city. The isolation of etiological agent was done by semi-quantitative method of inoculating the samples on the selective and differential media Eosin Methylene Blue (EMB) and MacConkey (MAC) Agar respectively. The isolated pathogen was identified by Gram staining, motility and biochemical tests. The antibiogram studies were carried out by Kirby-Bauer disc diffusion technique and ESβL production by double disk-diffusion test (DDDT) as per CLSI guidelines 2016. Out of 600 isolates screened 146 E. coli isolates were isolated which were multidrug resistant with 50% isolates being ESβL producers. All ESβL producing E. coli isolates were resistant to ceftazidime and exhibited higher level of resistance to Cephalothin, Erythromycin, Cotrimoxazole and Aztreonam. ESβL producing organisms limits the available treatment options. So, the prudent use of antibiotics will help in curing the disease without going for the costly drugs such as Carbapenems. We suggest nitrofurantoin, ofloxacin and tetracycline may be considered as drug of World Scientific News 102 (2018) 193-200 -194choice for the treatment in UTI patients. The ESβL production should be continuously monitored in the clinics and hospitals as to avoid the emergence of drug resistance.