Isolation, Identification and Partial Characterization of Bacteria Responsible for Hydrolysis of Tamarind Kernel Powder Based Gum

Tamarind kernel powder, popularly known as TKP, is a natural polysaccharide derived from the seeds of Tamarindus indica Linn. Tamarind is a very important tree commonly found in India and South East Asia. The gum prepared from the seeds of TKP finds wide applications in textile sizing and printing, paper binding, as additives in food and pharmaceutical products. In the textile industries this TKP based thickener is widely used in printing application. This TKP based gum is hydrolyzed after 48-72 hours. The hydrolysis is primarily attributed to the growth of bacteria and fungi. Bacterial isolates are isolated by various isolation methods. Isolates were identified by Gram staining, Biochemical tests and hydrolytic activity of the isolates on proteins, lipids and carbohydrates.