HPLC profile, antioxidant, antibacterial, and anti-proliferative activity of aerial parts of Ferula ovina (Boiss.) Boiss

K E Y W O R D S HPLC profile, antioxidant, antibacterial, and anti-proliferative activity of aerial parts of Ferula ovina (Boiss.) Boiss. MOHAMMAD HASSAN MOHAMMADI1 *, BEHNAM MAHDAVI2, ESMAEIL REZAEI-SERESHT2, SAHAR RIAHIFARSANI2 AND NAZANIN ENTEZARI3 1 Department of Chemistry,Islamic Azad University,Sabzevar Branch, , Sabzevar, Iran. 2 Department of Chemistry, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran. 3 Department of Anesthesiology, Faculty of Paramedical, Sabzevar University of Medical Sciences, Sabzevar, Iran Received: 12 May 2021 Revised: 22 July 2021 Accepted: 28 August 2021 ePublished: 28 September 2021 Corresponding author: Mohammad Hassan Mohammadi Tel: +98-51-44013319; Fax: +98-51-44012669 E-mail address: mhmohamady@iaus.ac.ir, doi: 10.30495/tpr.2021.1929063.1207 Antibacterial activity Antioxidant activity Ferrous ion chelating Ferula ovina (Boiss.) Boiss Radical scavenging activity acetate, ferocaulidin, conferone, and conferol acetate from roots of F. badrakema (Iranshahi et al., 2010b). Many of the Ferula species have been historically known as rich sources of aromatic resins. Numerous species of this genus have been used in traditional medicine for various organ disorders such as carminative, digestive, expectorant, sedative, anti-hysteric, laxative, aphrodisiac analgesic (Sahebkar and Iranshahi, 2010), antispasmodic (Sahebkar and Iranshahi, 2011) and antinociceptive (Bagheri et al., 2014). Ferula ovina (Boiss.) Boiss. is known by the Persian name of ''Koma''(Azarnivand et al., 2011). In contrast to the other Ferula species, the plant odor is not stinky due to the absence of sulfur-containing compounds. A previous study reported isolating stylosin, tschimgine as benzoic acid monoterpene derivatives, and ferutinin as a benzoic acid sesquiterpene derivative, from chloroform extract of the root of F. ovina Boiss (Iranshahi et al., 2010a). The essential oil of the aerial parts of the © 2021 Islamic Azad University, Shahrood Branch Press, All rights reserved. The areial parts of Ferula ovina (Boiss.) Boiss were extracted by methanol and then fractintioned with solvents that were differences in their polarity. The evaluation of the bioactivity of the plant extracts including antioxidant, antimicrobial and antiproliferative activity was carried out using various assays. The phenolic and flavonoid compound profile of the extract were established using HPLC analysis. The leaves showed the highest total phenolic content and radical scavenging activity; the flowers had the maximum amount of total flavonoid content, and the stems exhibited the maximum ferrous ion chelating ability. Among ten microorganisms, the plant extract prevented the growth of three strains, namely Pseudomonas aeruginosa, Acinetobacter baumannii, and Streptococcus pneumonia. The flower of F. ovina (Boiss.) Boiss shows the best anti-human cervical carcinoma property against HeLa cell lines. Gallic acid was found as the major phenolic compound in the plant extract according to the HPLC result.