Confirmation of routine identification of some bacterial species isolates from uti infected women using gene sequencing and 16 srrna techniques

The study had included isolation and identification of 140 samples of women attended Tikrit Teaching Hospital infected with urinary tract their ages were between (15-45) during the period from 1/4 / 2016 to 1/4/2017. This study clarified that (101) sample from the total samples showed bacterial growth in percentage (72.1%). It was clear according to the results of diagnosis that Escherichia coli had the highest proportion of isolations 39.6% from the total isolates followed by both Staphylococcus warnei and Klebsiella pneumoniae 11.8%. Enterococcus faecalis 9.9%, Proteus miribilis 6.9%. Pseudomonas aeruginosa 5.9%, both Streptococcus sobrinus and Pseudomonas stutzeri 4.9% Proteus vulgaris had the least percentage 3.96% by four from (101) isolates. The sensitivity of these isolates was tested by (10) antibiotic which included quinolones, aminoglycosides, betalactams and tetracyclines antibiotics. DNA has been extracted for 18 bacterial isolates (triplicate for each type) 3 isolates of Gram positive (Staphylococcus warnei, Streptococcus faecalis and streptococcus sobrinus) and Gram negative bacteria (E. coli, Klebsiella pneumonia and Proteus mirabilis) were included for molecular diagnosis after routine and Vitek 2 diagnosis. Results showed a single band with molecular weight 1411bp for 16sRNA gene for different isolates. The results of PCR confirmed by gene sequencing that chosen for molecular diagnosis were characterized by compatibility of the laboratory diagnosis with in molecular diagnosis and the isolates were compatible with results recorded in WHO gene bank.