Cytotoxic i ty Test ing of Gravio la ( Annona mur icata Linn . ) Leaf Extracts

Graviola (A. muricata) is a medicinal plant that has been widely used for the treatment of various diseases, such as rheumatoid, neuralgia, diabetes, hypertension, insomnia, parasitic infection, and cancer. However, there has been no report whether the extracts from A. muricata leaves have any potential to produce any adverse effect to the normal cells. The present study aims to evaluate the cytotoxicity of three extracts from A. muricata leaves using a cell proliferation assay in normal cell (Chang-Liver) and hepatocarcinoma cell (Hep G2) lines. The first extract (or Amm1) showed the most potent anti-cell proliferation effect on both cell lines with the IC50 value at 3.13 μg/ml in Chang-Liver cells and 11.06 μg/ml in Hep G2 cells. Amm2 extract had no effect on cell proliferation in both cell lines. Amm3 extract inhibited only Hep G2 cell proliferation at IC50 value at 20.56 μg/ml. These results suggest that the leaf extracts from A. muricata from the different processes of extraction exhibit the different effects on cell proliferation. Therefore, a concurrent consumption of A. muricata must be done with caution, particularly on type of extractions or sample preparations.