MOLECULAR CHARACTERIZATION AND CYTOPATHOLOGICAL STUDIES OF OLIVE LATENT VIRUS 1 (OLV-1) AND OLIVE LATENT VIRUS 2 1Om-Hashem

In a preliminary study for the assessment of the sanitary status of olive trees in Egypt during April and May in the two successive season 2014 symptomatic and asymptomatic ten olive cultivars from three diff Marsa Matrouh and Beheira. RNA was extracted and used directly for one step RT primer for each virus. Electron microscopy of sap extracts from olive infected leaves with the two viruses most commonly showed isometric particles consistent to Oleavirus Chenopodium quinoa of chloroplast membrane, deformation of chloroplast, tubular like structural, crossing the all wall and deformation and lysis of the nucleus. Nucleotide sequenc from the coat protein gene of OLV OLV-2 showed homology ranged from 98% to 94 % with Italy and Poland Isolates for OLV % to 94 % with USA and under study were identified as an isolates of OLV