In Vitro Profiling Of The Glutathione Reductase And Glutathione Peroxidase To Determine Low Dose Effects Of Bisphenol A On Glutathione System And Oxidative Stress

Background: Bisphenol-A (BPA) is an environmental xenoestrogen that has been studied for its impact on fertility in animals and humans. Growing evidence showed that Bisphenol-A can disrupt the cytochrome P450 enzymes activities, leading to generation of reactive oxygen species with endocrine Interference effect, and can affect multiple organs. The objective of the study was to investigate the effects of varying concentrations of BPA (0.05 1μM for 13 weeks) on glutathione reductase and on glutathione peroxidase in female rats. Method: The eleven experimental groups rats were administered; 0.05, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, and 1 mg of BPA/kg bw/day for 13weeks respectively and blood samples collected weekly. The glutathione reductase and glutathione peroxidase were assayed using ready-to-use reagent kits and autochemicalanalyser. Individual tests were carried out according to the kit specifications. Results: the results showed significant increase in glutathione reductase, but glutathione peroxidase and were decreases after BPA exposure. The findings suggest that BPA induce oxidative stress. Conclusion: BPA-induced changes in the oxidative state in rats and may be a potential cue of BPA-mediated disease conditions.