Prediction of trichomoniasis in women complaining vaginal discharge by different methods and determine some immunological markers

Trichomoniasis is the most prevalent non-viral sexually transmitted infection (STD) in the world caused by the vaginotropic extracellular protozoan parasite Trichomonas vaginalis. The present study was conducted in Babylon Province during the period from October, 2017 to June, 2018 in AL-Hilla Teaching Hospital and AL-Zahraa Hospital, to diagnosis Trichomonas vaginalis using two methods direct microbial examination and molecular method by polymerase chain reaction using specific primer pairs of T. vaginalis B-Tubulin 9/2 and study levels of some immunoglobulins and cytokines among infected women using immunological methods. A total of (125) blood and fluid vagina samples (110) from infected women and (15) from control women were taken from women aged between (15-45) years. Two vaginal swabs (one for direct microscopical examination and the other for molecular test) and a blood sample were collected from each woman. These women suffer from acute secretions, itching, yellowish green discharge and other symptoms. The results showed that the total frequency of infection by T. vaginalis was (20.9%) in microscope test, while in PCR technique was (13.6 %). The highest infected number and percentage of infected women was 24 (21.81%) at the age (26-35) years, while the lowest infected at the age (36-45) years was 5 (4.54%) respectively. Also the results revealed the frothy yellow to green discharge 31 (81.57%) while the malodor discharges gave 29 (23.6 8%). The results revealed a significant increase (P<0.01) in serum concentration of proinflammatory cytokines interleukin (8 and 12) and IgG and IgM in T. vaginalis infection patients in comparison with healthy control group.