Towards an Understanding of Ligand Induced Functional Conformational Changes of MexB Efflux Transporter
semanticscholar(2021)
Abstract
MexB, an RND-superfamily efflux pump, plays a vital role in
conferring resistance to cytotoxic molecules, including antibiotics, upon Gram-negative
bacteria. Although the principal mechanistic elements of switching between the
access, binding and extrusion conformers of the protomers of tripartite efflux
transporters have been described previously, details surrounding the further
mechanism that ends in either substrate extrusion or pump inhibition are
limited to observations based on the type of ligand bound to the transporter. A
central but missing link in the structure/mechanism relationship is a
description of how ligand-induced conformational changes in the presence of a
membrane and changing transporter protonation state lead to either substrate
extrusion or inhibition of the pump. Here, we report that differences in
conformational changes are governed by ligand binding to the transporter. The
current study describes important new information about ligand-induced
structural rearrangements and conformational changes of MexB in relation to the
protonation state of critical acidic residues. We used tetracycline (TET) as a
model substrate of MexB and phenylalanine-arginine beta-naphthylamide (PAβN) as a
model inhibitor of MexB to study the aforementioned conformational changes. This new information will contribute
to the design of new, effective and selective efflux pump inhibitors that could
play key roles in reversing antimicrobial resistance.
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