On the role of the Trypanosoma brucei HEN 1-family methyltransferase in siRNA 1 modification 2 3

On the role of the Trypanosoma brucei HEN1-family methyltransferase in siRNA 1 modification 2 3 Huafang Shi a , Rebecca L. Barnes a* , Nicholas Carriero b , Vanessa D. Atayde a* , Christian 4 Tschudi c# , Elisabetta Ullu a,d# 5 6 Department of Internal Medicine, School of Medicine, Yale University, New Haven, 7 Connecticut, USA a ; Department of Computer Science, Yale University, New Haven, 8 Connecticut, USA b ; Department of Epidemiology of Microbial Diseases, School of Public 9 Health, Yale University, New Haven, Connecticut, USA c ; Department of Cell Biology, School 10 of Medicine, Yale University, New Haven, Connecticut, USA d . 11 12 Running Head: Trypanosoma brucei HEN1 13 14 #Address correspondence to Christian Tschudi, christian.tschudi@yale.edu, or Elisabetta Ullu, 15 elisabetta.ullu@yale.edu. 16 *Present addresses: Rebecca L. Barnes, Department of Molecular Biology and Biotechnology, 17 University of Sheffield, Sheffield, United Kingdom; Vanessa D. Atayde, Department of 18 Microbiology and Immunology, McGill University, Montreal, Canada 19 20 H.S. and R.L.B. contributed equally to this work. 21 22 EC Accepts, published online ahead of print on 1 November 2013 Eukaryotic Cell doi:10.1128/EC.00233-13 Copyright © 2013, American Society for Microbiology. All Rights Reserved.