HAL (Le Centre pour la Communication Scientifique Directe)（2020）
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摘要
Executive summary – Box 1: In brief: Mitochondria and bioblasts .......................................... 2 1. Introduction ............................................................................................................................................................................. 8 2. Coupling states and rates in mitochondrial preparations ................................................................................... 8 2.1. Cellular and mitochondrial respiration ................................................................................................................. 8 2.1.1. Aerobic and anaerobic catabolism and ATP turnover Consortium Communication 2 of 44 Gnaiger E et al ― MitoEAGLE Task Group (2020) Bioenerg Commun 2020.1 2.1.2. Specification of biochemical dose and exposure 2.2. Mitochondrial preparations .................................................................................................................................... 10 2.3. Electron transfer pathways ..................................................................................................................................... 11 2.4. Respiratory coupling control .................................................................................................................................. 12 2.4.1. Coupling 2.4.2. Phosphorylation P» and P»/O2 ratio 2.4.3. Uncoupling 2.5. Coupling states and respiratory rates ................................................................................................................. 13 2.5.1. LEAK state 2.5.2. OXPHOS state 2.5.3. Electron transfer state 2.5.4. ROX state 2.5.5. Quantitative relations 2.5.6. The steady state 2.6. Classical terminology for isolated mitochondria ............................................................................................ 19 2.6.1. – 2.6.5. State 1 – State 5 2.7. Control and regulation .............................................................................................................................................. 21 3. What is a rate? – Box 2: Metabolic flows and fluxes: vectoral, vectorial, and scalar ............................. 21 4. Normalization of rate per sample................................................................................................................................. 23 4.1. Flow: per object ............................................................................................................................................................ 23 4.1.1. Count concentration 4.1.2. Flow per single object 4.2. Size-specific flux: per sample size .......................................................................................................................... 25 4.2.1. Mass concentration 4.2.2. Size-specific flux 4.3. Marker-specific flux: per mitochondrial content ............................................................................................. 26 4.3.1. Mitochondrial concentration and mitochondrial density 4.3.2. mt-Marker-specific flux 5. Normalization of rate per system ................................................................................................................................ 28 5.1. Flow: per chamber ...................................................................................................................................................... 28 5.2. Flux: per chamber volume ....................................................................................................................................... 28 5.2.1. System-specific flux 5.2.2. Advancement per volume 6. Conversion of units ............................................................................................................................................................ 30 7. Conclusions – Box 3: Recommendations for studies with mitochondrial preparations ...................... 31 References ............................................................................................................................................................................. 36 Authors (MitoEAGLE Task Group) – Author contributions .............................................................................. 41 Acknowledgements – Competing financial interests – Correspondence
