Genetic diversity determination in aquarium bred fresh water ornamental fish variety (poecilia species) using rapd molecular markers

Successful conservation of f ish such as Poecilia species depend on analysis of genetic signature and relations among f ish species and subspecies. Knowledge about the genetic signature and structure of Poecilia species needs to increase several times to enable us to conserve these economic f ish in the future. Many factors have contributed to the destruction of f ish habitats. Hydroelectric dams, water pollution and other environmental changes have resulted in the eradication of natural stocks. In the present study ten species of Mollies were collected from aquariums in Bangalore, the genomic DNA was extracted from f in tissues using standard protocol by phenol – chloroform method and using ten decamer oligo-nucleotides as single primers in Polymerase Chain Reaction (PCR), DNA f ingerprinting, dendrogram analysis and genetic similarity matrix were estimated, revealing variations between selected ten species of Poecilia. From the analyzed species three groups that were found to be 100% similar are f irst group of sample 1 and 2, second group of sample 3, 4, and 6 and third group of sample 7 and 8. In past few decades, molecular markers have evolved themselves and have totally changed our view of nature. In last ten years, advance technologies has supported greatly in determination of population diversity. The described approach holds great promise for further analyses and gives support to biodiversity maintenance as well as for conservation of genetic resources.