Authenticity Identification of the Traditional Chinese Herb Glycyrrhiza uralensis by Real-Time PCR and Loop-Mediated Isothermal Amplification

Background: Glycyrrhiza uralensis is a traditional Chinese herb, and sales volume of this herb is large. However, adulterant herbal materials threaten trade and consumer safety. Methods：A rapid detection system for the identification of G. uralensis was established using loop-mediated isothermal amplification (LAMP) and real-time fluorescence quantitative PCR (Real-Time PCR). The DNA was extracted and the G. uralensis primers were designed. LAMP and Real-Time PCR were performed to assessment the specificity of primers in species. The sensitivity of LAMP and Real-Time PCR was contrast by diluting DNA concentration gradient from 101 ng/μL to 10-5 ng/μL. Results: tThe results showed that LAMP and Real-Time PCR were specificity because G. uralensis were positive while similar species were not. The sensitivity of LAMP was similar to Real-Time PCR at DNA concentration of 10-4ng in 60 min. The results indicated that LAMP and Real-Time PCR are accurately, specificity and sensitivity in the Authenticity Identification of G. uralensis. Conclusions: LAMP is less time-consuming, convenient, and does not require expensive equipment. Thus, these findings suggested that a method system and standard of traditional Chinese herb market supervision should be established based on a DNA barcode sequence and LAMP for authenticity identification.