Expression Pattern of CircRNA in Naringin-induced Fate Dicision of BMSCs

Abstract Objective To investigate the effect of Naringin (NG) on the differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) in vitro. Methods BMSCs with different concentrations of NG were treated. The cell morphology, proliferation, toxicity analysis of each group, the osteogenic differentiation of BMSCs, and the expressions of markers related to osteogenic differentiation of BMSCs, osteogenic marker protein and CircRNAs were observed by Cell Counting Kit-8(CCK-8)method, Reverse Transcription-Polymerase Chain Reaction༈RT-PCR༉ method, etc. We used Miranda to predict the microRNA targets of CircRNAs and the targets of CircRNA-miRNA interaction, and constructed a CircRNA-microRNA co-expression network by analyzing the correlation between differentially expressed CircRNAs and microRNAs. The diagram of CircRNA-microRNA network was created with Cytoscape. Results The research results showed that NG of 1, 5, 10, 50, 100, 200µg/mL played an important role in accelerating the proliferation of BMSCs in vitro. It is found that osteocalcin (OCN), Sp7 transcription factor(SP7) and runt-related transcription factor 2 (RUNX2) were significantly enhanced in mRNA and protein expression levels. Furthermore, 633 CircRNAs showed an upward trend of expression, while 762 CircRNAs showed a downward trend of expression. Compared to those in the control group, the expression level of CircTll 1 decreased significantly (P < 0.01), the expression level of CircFkbp5 increased (P < 0.05), and the expression level of Circzbtbl6-2 and CircCped1 increased significantly (P < 0.001). In addition, we analyzed the target mRNAs of miR-342-3p by GO and Panther pathways and found that Circ14046, Circ10410 and Circ7511 were not only closely related to calcium signaling pathway, but also to the regulation of actin cytoskeleton involved in cell growth and differentiation. Conclusion This paper confirmed that Circ14046, Circ10410 and Circ7511 were related to osteogenic differentiation. NG may induce osteogenic differentiation of BMSCs through Circ14046/Circ10410/Circ7511 targeting miRNA-mRNA axis.