Identification of bioactive ingredients in sonchus oleraceus by hplc and gc/ms

This study aims to evaluate the successive extraction of the active ingredients and their anticancer activity of arial parts (stem, leaves and flowers) of S. oleaceous. Therefore, Sonchus oleraceus aerial parts were subjected to a successive extraction by using solvent of different polarities; each fraction was evaluated for its anticancer. Each active extract was farther biogided fractionated to determine and identify the active compound/s present in each sub-fraction. All the tested species were extracted using four solvents: hexane, ethyl acetate, methanol and water. Phenols, flavonoids and tannins were quantified for all extracts. The chemical analysis proved it to will be a good source of protein, fat and carbohydrates. The results showed that the percentages of moisture content (92.72%), ash content (15.14%), crude protein (25.94%), crude lipid (4.05%) and carbohydrate (54.87%) respectively of the S. oleraceus L. the highest content of total phenolic (TP), total flavonoid (TF) and total tannin (TT) was obtained by methanol fraction. The active ingredients were evaluated as well employing gas chromatography coupled to mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC). Gas chromatography mass spectrometry (GC-MS) data showed the identification of ten volatile compounds in hexane fraction of the Sonchus oleraceus, among them two were found to be the major components 9 Octadecenamide (CAS) 40.92% and 1 Hexacosanol 21.01%. The HPLC analysis of phenolic compounds confirmed that the water fraction of Sonchus oleraceus L detected high amounts of pyrogallol and chlorogenic respectively. The result showed that pronounced cytotoxic activity of cancer cells at 100 μg/ml in aqueous fraction in breast cancer (94.8%), while in Methanol extract in Colon Carcinoma (88%) On the other hand, ethyl acetate fraction of the Sonchus Oleraceus showed the highest percentage of colon cancer cells (82.6%) and breast cancer (80%), while the ethyl acetate extract in hepatocellular carcinoma cell line showed moderate effect (46.3%). Thus, our results highlight of Sonchus oleraceus L for its possible clinical use to oppose malignancy development against mostly breast and colon cell line as a bio agent in pharmaceutical industries.