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Determination of intracellular 2-hydroxyglutarate enantiomers using two-dimensional liquid chromatography

Takuma Ohtawa,Ayuna Hattori,Muneki Isokawa,Masashi Harada,Takashi Funatsu,Takahiro Ito,Makoto Tsunoda

Journal of Chromatography Open（2021）

Cited 3|Views1
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Abstract
•2-HG underwent lactonization before forming a fluorescent derivative with NBD-PZ.•A 2D liquid chromatography system with an ODS column and a chiral column established.•2-HG Enantiomers in U937 cells successfully quantified.•IDH mutant cells had more D-2-HG than wild-type cells.
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Key words
Cancer,Oncometabolite,Enantiomer separation,Fluorescence
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