A llobacillus salarius sp. nov., and Allobacillus saliphilus sp. nov., isolated from shrimp paste ( ka-pi ) in Thailand

Two strains of moderately halophilic, Gram-stain-positive and spore-forming rods, designated as SKP4-8 T and SKP8-2 T isolated from shrimp paste ( Ka-pi ), were taxonomic studied based on polyphasic approach. Strain SKP4-8 T grew at pH 6.0–9.0 (optimum 7.0), at 25–45 °C (optimum 37 °C) and in 1–16% (w/v) NaCl (optimum 5–10%). Strain SKP8-2 T grew at pH 6.0–9.0 (optimum 8.0), at 25–45 °C (optimum 37 °C) and in 0–20% (w/v) NaCl (optimum 3–10%). The strains contained meso -diaminopimelic acid in cell-wall peptidoglycan and the major menaquinone was MK-7. Strain SKP4-8 T contained iso-C 15:0 , anteiso-C 15:0 and iso-C 17:0 ; and strain SKP8-2 T contained anteiso-C 15:0 , iso-C 15:0 , iso-C 16:0 and antesio-C 17:0 as major cellular fatty acids. Phosphatidylglycerol, diphosphatidylglycerol, unknown phospholipids and an unknown glycolipid were detected as major polar lipids. On the basis of 16S rRNA gene sequence analysis, strains SKP4-8 T and SKP8-2 T belonged to the genus Allobacillus and were closely related to Allobacillus halotolerans LMG 24826 T with 98.8% and 99.3% similarity, respectively. The comparative genome analysis based on average nucleotide identity (ANI) and digital DNA–DNA hybridization revealed that both strains showed the values below 95 and 70%, from each other and from Allobacillus halotolerans LMG 24826 T , respectively. Based on the data from this polyphasic study, strains SKP4-8 T and SKP8-2 T represent novel species of the genus Allobacillus and the name Allobacillus salarius sp. nov. was proposed for SKP4-8 T (= KCTC 33905 T = LMG 30016 T = TISTR 2499 T ); and Allobacillus saliphilus sp. nov. for SKP8-2 T (= KCTC 33906 T = LMG 29682 T = TISTR 2558 T ).