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Circulating Cell-Free DNA (ccfDNA) Isolation from Patients with Diffuse Large B-Cell Lymphoma (DLBCL): Comparative Analysis of Commercial Kits

Karolliny Silva de Oliveira,Hebert Fabricio Culler,Débora Levy,José Roberto Assis Filho,Daniel Silva Nogueira,Lis Vilela de Almeida,Luiz Henrique da Silva, Fernando Luiz Affonso Fonseca,Sarah Isabel Pinto Monteiro do Nascimento Alves,Luis Alberto de Padua Covas Lage,Juliana Pereira

Blood（2021）

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Abstract
Introduction: Isolation of circulating cell-free DNA (ccfDNA) and circulating tumor DNA (ctDNA) are useful methodologies for studies of neoplastic genetic material and can provide information about cancer heterogeneity, prognosis and minimal residual disease (MRD) [1,2]. Therefore, the recovery of ccfDNA in sufficient quantity and adequate purity is crucial to provide accurate and reproducible results. In this study, we investigate the efficiency of four different commercial kits to recover short fragments of DNA compatible with the ccfDNA.
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Key words
ccfdna,cell-free,b-cell
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