Deterministic Cell Separation Recovers >2-Fold T Cells, and More Naive T Cells, for Autologous Cell Therapy As Compared to Centrifugally Prepared Cells

CAR-T autologous cell therapies are delivering impressive results in the clinic. However, there are still significant manufacturing challenges impeding the rapid adoption of these advanced therapies. On the first day of cell processing, most manufacturing approaches require ~5 steps (~4 hours) to obtain a white blood cell (WBC) preparation sufficiently depleted of red blood cells (RBCs) for T-cell selection and activation steps; and involves large cell losses and a great deal of inconsistency. Here we present a single-step procedure that yields >2 fold more cells that centrifugal processing with comparable or better quality in <1 hour.