Transcriptome analysis of potential flocculation-related genes in Streptomyces sp. hsn06 with flocculation activity on Chlorella vulgaris biomass
Archives of Microbiology(2021)
Abstract
Chlorella vulgaris is a biomass energy provider with promising potential to help alleviate the energy crisis. Streptomyces sp. hsn06, as an actinomycete, can harvest C. vulgaris biomass safely and efficiently through flocculation activity, and proteins contribute greatly to the flocculation effect. However, potential flocculation protein-related genes are unclear. The mycelia of strain hsn06 after culture with glucose as the sole carbon source exhibited significantly higher flocculation activity as well as higher protein contents than those cultured with starch as the carbon source. To further explore the flocculation mechanism, the mycelia of strain hsn06 with distinct flocculation activities after culture with different carbon sources were examined by transcriptome analysis. We found that 403 genes were differentially up-regulated in mycelia cultured with glucose, compared to those cultured with starch as the carbon source. Five significantly differentially expressed protein-related genes were determined and confirmed by qRT-PCR, which indicated that three of the selected genes were potential flocculation-related genes. These results advance our understanding of potential flocculation-related genes during the harvesting of microalgal biomass.
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Key words
Streptomyces sp. hsn06,Chlorella vulgaris biomass,Transcriptome analysis,qRT-PCR,Potential flocculation-related genes
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