A sequel study on the occurrence of Tomato spotted wilt virus (TSWV) in cut-chrysanthemum by DAS-ELISA using recombinant nucleocapsid protein to produce polyclonal antiserum

•Nucleocapsid protein of TSWV was expressed in bacterial expression system using rDNA technology.•N protein was confirmed by western blot analysis using anti-rabbit immunoglobulin (IgG) tagged with Alexa fluor plus 800 fluorescent dye.•Polyclonal antiserum was raised using expressed N protein of TSWV.•DAS-ELISA reacted specifically to naturally infected chrysanthemum without any cross-reaction with other tospovirus.•Infection of TSWV in chrysanthemum stem cuttings was screened using the developed TSWV antiserum in large numbers from various locations.