Generation and utility of a single-chain fragment variable monoclonal antibody platform against a baculovirus expressed recombinant receptor binding domain of SARS-CoV-2 spike protein

Reda Salem,Alaa A El-Kholy,Fatma R Waly,Dalia Ayman,Aya Sakr, Mai Hussein

Molecular Immunology（2022）

引用 6|浏览3

暂无评分

摘要

•Cloning and expression the RBD of SARS-CoV-2 in Baculovirus Expression Vector System.•Recombinant RBD has antigenic and immunogenic properties.•Recombinant RBD exhibiting specific reactivity to anti-SARS-CoV-2 antibody.•Constructing a scFv phage display library against rRBD.•Verification the scFv: accurate structure, authentic immunoreactivity, and specificity.

查看译文

关键词

SARS-CoV-2,Murine scFv antibodies,Recombinant RBD,Baculovirus expression vector system,Phage display,COVID-19

AI 理解论文

溯源树

样例

生成溯源树，研究论文发展脉络

Chat Paper

正在生成论文摘要